Departamento de Ciencias Alimentos y Nutrición

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http://repositorioabierto.uantof.cl/handle/uantof/229

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Examinando Departamento de Ciencias Alimentos y Nutrición por Autor "Carlos Vílchez"

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  • Miniatura
    Ítem
    Determining the Potential of Haematococcus pluvialis Oleoresin as a Rich Source of Antioxidants
    (2019) Mari Carmen Ruiz Domínguez; Carolina Espinosa; Adrián Paredes; Jenifer Palma; Carolina Jaime; Carlos Vílchez; Pedro Cerezal
    Haematococcus pluvialis is known to be a natural source of antioxidants for numerous applications. In this study, an oleoresin rich in carotenoids extracted by supercritical CO2 treatment of H. pluvialis was extensively characterized for its antioxidant capacity. Carotenoid content, fatty acid profile, total phenol content, antioxidant capacity, and viscosity of the oleoresin were determined with the aim of ascertaining the potential of the oleoresin in terms of its antioxidant content for food applications. The oleoresin contained 96.22 mg/g of total astaxanthin (which includes free astaxanthin and astaxanthin esters) and mostly included unsaturated fatty acids (~78% of total fatty acids). High total phenol content and ferric reducing antioxidant potential indicated high antioxidant capacity, but oxygen radical absorbance capacity was lower compared to the oleoresin samples obtained from other species. The oleoresin was a non-Newtonian fluid since it had shear-thinning (pseudoplastic) and shear-thickening (dilatant) flow. Therefore, the H. pluvialis oleoresin is a potential alternative in developing functional ingredients for designing healthy food products. To the best of our knowledge, this is the first study that has reported an extensive characterization of the antioxidant properties of a microalgal oleoresin obtained by means of supercritical CO2 fluid extraction.
  • Miniatura
    Ítem
    Mixotrophic growth of a highly acidic habitat microalga for production of valuable fatty acids
    (2024) María Robles; Carol Ostojic; Mari Carmen Ruiz‑Domínguez; María Cuaresma; Carlos Gonzalo; Virginia Obregón; Juan Luis Fuentes; Alejandra Bartolomé; Carlos Vílchez
    Photoautotrophic algal cultivation is challenging due to the shadowing effect produced by an increase in the number of cells; under such circumstances, mixotrophic growth might be an efficient alternative. We grew cultures of the autochthonous acidotolerant microalga Elliptochloris sp. on crude technical glycerin or glucose bubbled with either only air or air containing 2.5 % (v/v) CO2. We found that CO2 strongly influenced the production of Elliptochloris sp., as higher growth occurred in mixotrophy with CO2-enriched air compared to that with only air. Mixotrophy with CO2-enriched air allowed to reach higher biomass productivities and facilitated an increase in the relative abundance of saturated fatty acids. Nevertheless, mixotrophy with only air resulted in an increase of both saturated and unsaturated fatty acids, but biomass productivities were lower. These results, along with the limited biological contamination facilitated by low pH, suggest that this microalga might be attractive for large-scale production within the circular economy model.