Examinando por Autor "Gómez Silva, Benito"

Mostrando 1 - 3 de 3
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    Ítem
    Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands
    (MDPI, 2020) Galetović, Alexandra ; Azevedo, Joana; Castelo Branco, Raquel; Oliveira, Falvio; Gómez Silva, Benito ; Vasconselos, Vitor
    Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. Llayta and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin and -N-methylamino-L-alanine—BMAA) usingmolecular and chemicalmethods. All isolates were free of target genes involved in toxin biosynthesis. Only DNA from Llayta amplified the mcyE gene. Presence of microcystin-LR and BMAA in Llayta extracts was discarded by LC/MS analyses. The analysed Llayta colonies have an incomplete microcystin biosynthetic pathway and are a safe food ingredient.
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    Ítem
    Draft Genome Sequence of Staphylococcus sciuri Strain LCHXa, a Lithium-Tolerant Bacterium Isolated from Laguna Chaxa, Salar de Atacama, Chile.
    (American Society for Microbiology, 2019) Vilo, Claudia ; Salazar Ardiles, Camila; Caimanque, Tamara N.; Dong, Qunfeng ; Flores, Nataly ; Galetović, Alexandra ; Araya, Jorge E. ; Gómez Silva, Benito
    A Gram-positive, coagulase-negative, novobiocin resistant, and lithiumtolerant bacterium was isolated from Salar de Atacama. Strain LCHXa is closely related to Staphylococcus sciuri. Its genome is 3,013,090 bp long and contains 2,551 predicted protein genes. We observed 58 genes associated with stress response and 17 genes linked to osmoregulation, mainly related to glycine betaine metabolism.
  • Miniatura
    Ítem
    Uncovering Hidden Microbial Diversity in Nitrate/Iodide Deposits (NIDs) in the Domeyko District, Atacama Desert, Chile
    (MDPI, 2024) Cortés, Mayra; Avendaño, Priscilla; Encalada, Olga; Salazar Ardiles, Camila; Andrade, David C.; Gómez Silva, Benito; Contreras, Daniel; Toro, Norman; Arias, Dayana; Escudero, Lorena V.
    Unique worldwide, nitrate/iodine deposits (NIDs) are located along a 700 km geological belt in the Atacama Desert, Chile. They serve as the primary source of mineral ores for the extraction of iodine, sodium, and potassium nitrates. NIDs have been relatively underexplored from a biological perspective. To address this, we collected sixteen soil samples from abandoned mines in Oficinas Pissis and Savona for chemical, mineralogical, and metagenomic analyses. The soils primarily consisted of halite and darapskite, with only one sample being predominantly composed of thenardite. Deliquescence and water activity measurements yielded values ranging from 0.02% to 0.40% and 0.47 to 0.62, respectively. To investigate the presence, identification, relative abundance, and diversity of microbial life in NID soils, we employed MiSeq high-throughput sequencing and bioinformatic tools. The dominant phyla observed were Firmicutes and Proteobacteria, with Actinobacteria and Cyanobacteria being predominant in two soil samples. Furthermore, we detected nitrate/perchloratereducing bacterial activity in enriched cultures from the soil samples. This study sheds light on the resilience of microbial life in the Atacama Desert NIDs, providing compelling evidence for its existence and offering insight into factors that could facilitate it within this unique environment.