Examinando por Autor "Cristina Dorador"

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    Protist communities of microbial mats from the extreme environments of five saline Andean lagoons at high altitudes in the Atacama Desert
    (2024) Eduardo Acosta; Frank Nitsche; Cristina Dorador; Hartmut Arndt
    Introduction:Heterotrophic protists colonizing microbial mats have received little attention over the last few years, despite their importance in microbial food webs. A significant challenge originates from the fact that many protists remain uncultivable and their functions remain poorly understood.Methods:Metabarcoding studies of protists in microbial mats across high-altitude lagoons of different salinities (4.3–34 practical salinity units) were carried out to provide insights into their vertical stratification at the millimeter scale. DNA and cDNA were analyzed for selected stations.Results:Sequence variants classified as the amoeboid rhizarian Rhogostoma and the ciliate Euplotes were found to be common members of the heterotrophic protist communities. They were accompanied by diatoms and kinetoplastids.Correlation analyses point to the salinity of the water columnas a main driver influencing the structure of the protist communities at the five studied microbial mats. The active part of the protist communities was detected to be higher at lower salinities (<20 practical salinity units).Discussion:We found a restricted overlap of the protist community between the different microbial mats indicating the uniqueness of these different aquatic habitats. On the other hand, the dominating genotypes present in metabarcoding were similar and could be isolated and sequenced in comparative studies(Rhogostoma, Euplotes,Neobodo). Our results provide a snapshot of the unculturable protist diversity thriving the benthic zone of ive athalossohaline lagoons across the Andean plateau.
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    Secondary metabolites with antimicrobial activity produced by thermophilic bacteria from a high-altitude hydrothermal system
    (2024) Coral Pardo Esté; Johanna Cortés; Juan Castro Severyn; Vilma Pérez; Karem Henriquez Aedo; Fabian Cuadros; Carolina Yañez; Sara Cuadros Orellana; Cristina Dorador; Veronica Molina; Yoanna Eissler; Pablo Paquis; Wade H. Jeffrey; Patricia Pozo; Pablo A. Pérez; Martha B. Hengst
    Thermophilic microorganisms possess several adaptations to thrive in high temperature, which is reflected as biosynthesis of proteins and thermostable molecules, isolation and culture represent a great methodological challenge, therefore High throughput sequencing enables screening of the whole bacterial genome for functional potential, providing rapid and cost-effective information to guide targeted cultures for the identification and characterization of novel natural products. In this study, we isolated two thermophilic bacterial strains corresponding to Bacillus LB7 and Streptomyces LB8, from the microbial mats in the Atacama Desert. By combining genome mining, targeted cultures and biochemical characterization, we aimed to identify their capacity to synthesize bioactive compounds with antimicrobial properties. Additionally, we determined the capability to produce bioactive compounds under controlled in vitro assays and detected by determining their masses by Thin-Layer Chromatography/Mass Spectrometry (TLC/MS). Overall, both isolates can produce antimicrobial (e.g., Myxalamide C by-product) and antioxidants (e.g. Dihydroxymandelic Acid, Amide biotine and Flavone by-products) compounds. Bacillus LB7 strain possesses a more diverse repertoire with 51.95% of total metabolites unmatched, while Streptomyces LB8 favors mainly antioxidants, but has over 70% of unclassified compounds, highlighting the necessity to study and elucidate the structure of novel compounds. Based on these results, we postulate that the uncultured or rare cultured thermophiles inhabiting high-altitude hydrothermal ecosystems in the Atacama Desert offer a promising opportunity to the study of novel microbial bioactive compounds.
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    Shared governance in the plant holobiont and implications for one health
    (2024) Gabriele Berg; Cristina Dorador; Dilfuza Egamberdieva; Joel E. Kostka; Choong-Min Ryu; Birgit Wassermann
    The holobiont Holobiont theory is more than 80 years old, while the importance of microbial communities for plant holobionts was already identified by Lorenz Hiltner more than a century ago. Both concepts are strongly supported by results from the new field of micr obiome r esear c h. Here , w e present ecolog ical and g enetic featur es of the plant holobiont that underpin principles of a shar ed governance between hosts and microbes and summarize the relevance of plant holobionts in the context of global c hange . Moreover, we unco ver kno wledge gaps that arise when inte gr ating plant holobionts in the broader perspective of the holobiome as well as one and planetary health concepts. Action is needed to consider interacting holobionts at the holobiome scale, for prediction and control of microbiome function to improve human and environmental health outcomes.
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    The Chilean COVID-19 Genomics Network Biorepository: A Resource for Multi-Omics Studies of COVID-19 and Long COVID in a Latin American Population
    (2024) Iskra A. Signore; Gerardo Donoso; Pamela Bocchieri; Eduardo A. Tobar-Calfucoy; Cristian E. Yáñez; Laura Carvajal-Silva; Andrea X. Silva; Carola Otth; Claudio Cappelli; Héctor Valenzuela Jorquera; Daniela Zapata Contreras; Yolanda Espinosa Parrilla; Paula Zúñiga Pacheco; Macarena Fuentes Guajardo; Virginia A. Monardes Ramírez; Pia Kochifas Velasquez; Christian A. Muñoz; Cristina Dorador; Jonathan García Araya; Claudia P. Campillay Véliz; Cesar Echeverria; Rodolfo Alejandro Santander; Leslie C. Cerpa; Matías F. Martínez; Luis Abel Quiñones; Eduardo Roberto Lamoza Galleguillos; Juan Saez Hidalgo; Estefanía Nova-Lamperti; Sergio Sanhueza; Annesi Giacaman; Gerardo Acosta Jamett; Cristóbal Verdugo; Anita Plaza; Claudio Verdugo; Carolina Selman; Ricardo Alejandro Verdugo; Alicia Colombo
    Although a lack of diversity in genetic studies is an acknowledged obstacle for personalized medicine and precision public health, Latin American populations remain particularly understudied despite their heterogeneity and mixed ancestry. This gap extends to COVID-19 despite its variability in susceptibility and clinical course, where ethnic background appears to influence disease severity, with non-Europeans facing higher hospitalization rates. In addition, access to high-quality samples and data is a critical issue for personalized and precision medicine, and it has become clear that the solution lies in biobanks. The creation of the Chilean COVID-19 Biorepository reported here addresses these gaps, representing the first nationwide multicentric Chilean initiative. It operates under rigorous biobanking standards and serves as one of South America’s largest COVID cohorts. A centralized harmonization strategy was chosen and included unified standard operating procedures, a sampling coding system, and biobanking staff training. Adults with confirmed SARS-CoV-2 infection provided broad informed consent. Samples were collected to preserve blood, plasma, buffy coat, and DNA. Quality controls included adherence to the standard preanalytical code, incident reporting, and DNA concentration and absorbance ratio 260/280 assessments. Detailed sociodemographic, health, medication, and preexisting condition data were gathered. In five months, 2262 participants were enrolled, pseudonymized, and sorted by disease severity. The average Amerindian ancestry considering all participant was 44.0% [SD 15.5%], and this value increased to 61.2% [SD 19.5%] among those who self-identified as Native South Americans. Notably, 279 participants self-identified with one of 12 ethnic groups. High compliance (>90%) in all assessed quality controls was achieved. Looking ahead, our team founded the COVID-19 Genomics Network (C19-GenoNet) focused on identifying genetic factors influencing SARS-CoV-2 outcomes. In conclusion, this bottom-up collaborative effort aims to promote the integration of Latin American populations into global genetic research and welcomes collaborations supporting this endeavor. Interested parties are invited to explore collaboration opportunities through our catalog, accessible online.