Examinando por Autor "Eckart D. Gundelfinger"

Mostrando 1 - 1 de 1
  • Miniatura
    Ítem
    Serine–Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Sca olding Protein CAST1/ERC2
    (2019) Duxan Arancibia; Matias Lira; Yocelin Cruz; Daniela P. Barrera; Carolina Montenegro Venegas; Juan A. Godoy; Craig C. Garner; Nibaldo C. Inestrosa; Eckart D. Gundelfinger; Pedro Zamorano; VivianaI.Torres
    Neurons release neurotransmitters at a specialized region of the presynaptic membrane, the active zone (AZ), where a complex meshwork of proteins organizes the release apparatus. The formation of this proteinaceous cytomatrix at the AZ (CAZ) depends on precise homo- and hetero-oligomerizations of distinct CAZ proteins. The CAZ protein CAST1/ERC2 contains four coiled-coil (CC) domains that interact with other CAZ proteins, but also promote self-assembly, which is an essential step for its integration during AZ formation. The self-assembly and synaptic recruitmentoftheDrosophilaproteinBruchpilot(BRP),apartialhomologofCAST1/ERC2,ismodulated by the serine-arginine protein kinase (SRPK79D). Here, we demonstrate that overexpression of the vertebrate SRPK2 regulates the self-assembly of CAST1/ERC2 in HEK293T, SH-SY5Y and HT-22 cells and the CC1 and CC4 domains are involved in this process. Moreover, the isoform SRPK2 forms a complex with CAST1/ERC2 when co-expressed in HEK293T and SH-SY5Y cells. More importantly, SRPK2 is present in brain synaptic fractions and synapses, suggesting that this protein kinase might control the level of self-aggregation of CAST1/ERC2 in synapses, and thereby modulate presynaptic assembly.