Examinando por Autor "Qunfeng Dong"

Mostrando 1 - 2 de 2
  • Miniatura
    Ítem
    Draft Genome Sequence of Staphylococcus sciuri Strain LCHXa, a Lithium-Tolerant Bacterium Isolated from Laguna Chaxa, Salar de Atacama, Chile.
    (2019) Claudia Vilo; Camila Salazar Ardiles; Tamara N. Caimanque,; Qunfeng Dong; Nataly Flores; Alexandra Galetović; Jorge E. Araya; Benito Gómez Silva
    A Gram-positive, coagulase-negative, novobiocin resistant, and lithiumtolerant bacterium was isolated from Salar de Atacama. Strain LCHXa is closely related to Staphylococcus sciuri. Its genome is 3,013,090 bp long and contains 2,551 predicted protein genes. We observed 58 genes associated with stress response and 17 genes linked to osmoregulation, mainly related to glycine betaine metabolism.
  • Miniatura
    Ítem
    Metagenomics of Atacama Lithobiontic Extremophile Life Unveils Highlights on Fungal Communities, Biogeochemical Cycles and Carbohydrate-Active Enzymes
    (2019) Benito Gómez-Silva; Claudia Vilo-Muñoz; Alexandra Galetović; Qunfeng Dong; Hugo G. Castelán Sánchez; Yordanis Pérez Llano; María del Rayo Sánchez Carbente; Sonia Dávila Ramos; Nohemí Gabriela Cortés López; Liliana Martínez Ávila; Alan D. W. Dobson; Ramón Alberto Batista García
    Halites, which are typically found in various Atacama locations, are evaporitic rocks that are considered as micro-scaled salterns. Both structural and functional metagenomic analyses of halite nodules were performed. Structural analyses indicated that the halite microbiota is mainly composed of NaCl-adapted microorganisms. In addition, halites appear to harbor a limited diversity of fungal families together with a biodiverse collection of protozoa. Functional analysis indicated that the halite microbiome possesses the capacity to make an extensive contribution to carbon, nitrogen, and sulfur cycles, but possess a limited capacity to fix nitrogen. The halite metagenome also contains a vast repertory of carbohydrate active enzymes (CAZY) with glycosyl transferases being the most abundant class present, followed by glycosyl hydrolases (GH). Amylases were also present in high abundance, with GH also being identified. Thus, the halite microbiota is a potential useful source of novel enzymes that could have biotechnological applicability. This is the first metagenomic report of fungi and protozoa as endolithobionts of halite nodules, as well as the first attempt to describe the repertoire of CAZY in this community. In addition, we present a comprehensive functional metagenomic analysis of the metabolic capacities of the halite microbiota, providing evidence for the first time on the sulfur cycle in Atacama halites.