HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells

Vista sencilla de ítem
dc.contributor.authorFarias, Monica A
dc.contributor.authorCancino, Felipe A
dc.contributor.authorNavarro, Areli J
dc.contributor.authorDuarte, Luisa F
dc.contributor.authorSoto, Abel A
dc.contributor.authorTognarelli, Eduardo I
dc.contributor.authorRamm, Maximiliano J
dc.contributor.authorAlarcon-Zapata, Barbara N
dc.contributor.authorCordero, Jose
dc.contributor.authorMartin, Sergio San
dc.contributor.authorAgurto-Munoz, Cristian
dc.contributor.authorRetamal-Diaz, Angello
dc.contributor.authorRiedel, Claudia A
dc.contributor.authorBarrera, Nelson P
dc.contributor.authorBustamante, Luis
dc.contributor.authorBueno, Susan M
dc.contributor.authorKalergis, Alexis M
dc.contributor.authorGonzalez, Pablo A
dc.date.accessioned2026-08-28T01:24:32Z
dc.date.available2026-08-28T01:24:32Z
dc.date.issued2025
dc.description.abstractHerpes simplex virus type 1 (HSV-1) significantly impairs dendritic cell (DC) function, ultimately eliciting the death of these cells. Here, we sought to assess whether HSV-1 modulates lipid metabolism in mouse DCs as a mechanism of immune evasion. For this, we performed RT-qPCR gene arrays with ingenuity pathway analysis (IPA), RNA sequencing (RNA-seq) and gene set enrichment analysis (GSEA), confocal microscopy, transmission electron microscopy, ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) analysis, pharmacological inhibition of eight lipid-metabolism-related enzymes in HSV-1-infected DCs, co-cultures between virus-specific transgenic CD4+ and CD8+ T cells and HSV-1-infected DCs, and in vivo assays with mice. We found that HSV-1 significantly alters lipid metabolism in DCs and induces lipid droplet (LD) accumulation in these cells. Pharmacological inhibition of two particular lipid metabolism enzymes was found to partially restore DC function. Overall, these results suggest that lipid metabolism plays an important role in the impairment of DC function by HSV-1.
dc.description.sponsorshipAgencia Nacional de Investigacion y Desarrollo (ANID) - Millennium Science Initiative Program - Millennium Institute on Immunology and Immunotherapy (MIII) [ICN2021_045, ICN09_016, P09/016-F]; ANID FONDECYT [1240971, 1231905, 1231851, 11241567, 11181200, 3210473]; Regional Government of Antofagasta through the Innovation Fund for Competitiveness FIC-R 2017 [30488811-0]; ANID [21191390, 21251401, 21211300, 21241486, 21241472]; Fondequip [EQM 170023]
dc.identifier.doi10.1016/j.isci.2025.112441
dc.identifier.issn2589-0042
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/717
dc.language.isoen
dc.publisherCell Press
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceiScience
dc.titleHSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells
dc.typeArticle
oaire.citation.volume28
oaire.citationIssue5
organization.identifier.rorhttps://ror.org/04eyc6d95
organization.legalNameUniversidad de Antofagasta
uantof.identificator.centerCentro de Investigación en Inmunología y Biotecnología Biomédica de Antofagasta
uantof.identificator.departmentDepartamento de Biotecnología
uantof.identificator.facultyFacultad de Ciencias del Mar y Recursos Biológicos
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
10.1016_j.isci.2025.112441.pdf
Tamaño:
8.73 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed to upon submission
Descripción:
Descargar
Colecciones
Departamento de Biotecnología