Rapid detection of enterobacteria inwastewater treated by microalgal consortiausing loop-mediated isothermalamplification (LAMP)

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dc.contributor.authorHenry Cameron
dc.contributor.authorJazmín Bazaes
dc.contributor.authorClaudia Sepúlveda
dc.contributor.authorCarlos Riquelme
dc.date.accessioned2026-04-23T19:49:37Z
dc.date.available2026-04-23T19:49:37Z
dc.date.issued2024
dc.description.abstractIn the present study, nine Enterobacteriaceae species present in wastewater were isolated and identified, and loop-mediated isothermal amplification (LAMP) was developed for the detection of Enterobacteriaceae by designing primers based on themcr-1, KPC, OXA-23, and VIM genes, which are recognized markers of antimicrobial resistance (AMR) transmission during microalgal bioremediation treatment. The developed assays successfully detected four strains positive for mcr-1gene-asociated resistance (Acinetobacter baylyi,Klebsiella pneumoniae,Morganellamorganii, andSerratia liquefaciens), three strains for KPC gene-associated resistance(Acinetobacter sp.,Escherichia coli15499, and Morganella morganii), seven strains for OXA-23 gene-associated resistance (Acinetobacter baylyi,Enterobacterhormaechi,Enterobacter cloacae,Escherichia coli15922,Escherichia coli51446,Morganella morganii, and Serratia liquefaciens), and three strains for resistance to the VIM gene-associated resistance (Acinetobacter baylyi,Acinetobactersp., and Enterobacter hormaechi) from a single colony. A reduction in microbiological load of93.6% was achieved at 15 colony-forming units (CFU) mL-1, utilizing EMB agar andLAMP values of 0.142 ± 0.011 for the mcr-1 gene, 0.212 ± 0.02 for the KPC gene,0.233 ± 0.006 for the OXA-23 gene, and 0.219 ± 0.035 for the VIM gene.Furthermore, bioremediation efficiency values of 71.6% and 75% for total nitrogen and phosphorus, respectively, were observed at 72 h of treatment in open pond microalgal remediation systems (MRS). This study demonstrated that the LAMP technique is faster and more sensitive than traditional detection methods, such as CFU, for Enterobacteriaceae. Consequently, this method may be considered for the detection of microbiological quality indicators within the water treatment industry
dc.description.sponsorshipANID IT21I0061
dc.identifier.doi10.7717/peerj.18305
dc.identifier.issn21678359
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/700
dc.language.isoen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourcePeerJ
dc.titleRapid detection of enterobacteria inwastewater treated by microalgal consortiausing loop-mediated isothermalamplification (LAMP)
dc.typeArticle
oaire.citation.volume12
organization.identifier.rorUniversidad de Antofagasta
uantof.identificator.centerCentro de Bioinnovación
uantof.identificator.facultyFacultad de Ciencias del Mar y Recursos Biológicos
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