Serine–Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Sca olding Protein CAST1/ERC2

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dc.contributor.authorArancibia, Duxan
dc.contributor.authorLira, Matias
dc.contributor.authorCruz, Yocelin
dc.contributor.authorBarrera, Daniela P.
dc.contributor.authorMontenegro Venegas, Carolina
dc.contributor.authorGodoy, Juan A.
dc.contributor.authorGarnen, Craig C.
dc.contributor.authorInestrosa, Nibaldo C.
dc.contributor.authorGundelfinger, Eckart D.
dc.contributor.authorZamorano, Pedro
dc.contributor.authorTorres, Viviana I.
dc.date.accessioned2026-01-15T15:51:28Z
dc.date.available2026-01-15T15:51:28Z
dc.date.issued2019
dc.description.abstractNeurons release neurotransmitters at a specialized region of the presynaptic membrane, the active zone (AZ), where a complex meshwork of proteins organizes the release apparatus. The formation of this proteinaceous cytomatrix at the AZ (CAZ) depends on precise homo- and hetero-oligomerizations of distinct CAZ proteins. The CAZ protein CAST1/ERC2 contains four coiled-coil (CC) domains that interact with other CAZ proteins, but also promote self-assembly, which is an essential step for its integration during AZ formation. The self-assembly and synaptic recruitmentoftheDrosophilaproteinBruchpilot(BRP),apartialhomologofCAST1/ERC2,ismodulated by the serine-arginine protein kinase (SRPK79D). Here, we demonstrate that overexpression of the vertebrate SRPK2 regulates the self-assembly of CAST1/ERC2 in HEK293T, SH-SY5Y and HT-22 cells and the CC1 and CC4 domains are involved in this process. Moreover, the isoform SRPK2 forms a complex with CAST1/ERC2 when co-expressed in HEK293T and SH-SY5Y cells. More importantly, SRPK2 is present in brain synaptic fractions and synapses, suggesting that this protein kinase might control the level of self-aggregation of CAST1/ERC2 in synapses, and thereby modulate presynaptic assembly.
dc.description.sponsorshipFONDECYT 1110944 BMBF 20150065 Basal Center of Excellence in Aging and Regeneration AFB 170005 FONDECYT 1160724 Sociedad Química y Minera de Chile Programa de Iniciación en Investigación para investigadores Jóvenes de la Universidad de Antofagasta D446-2015 to D.A.
dc.identifier.doi10.3390/cells8111333
dc.identifier.issn20734409
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/613
dc.language.isoen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceCells
dc.titleSerine–Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Sca olding Protein CAST1/ERC2
dc.typeArticle
oaire.citation.volume8
organization.identifier.rorhttps://ror.org/04eyc6d95
organization.legalNameUniversidad de Antofagasta
uantof.identificator.departmentDepartamento Biomédico
uantof.identificator.facultyFacultad de Ciencias de la Salud
uantof.identificator.instituteInstituto Antofagasta
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