In Silico Characterization of Calcineurin from Pathogenic Obligate Intracellular Trypanosomatids: Potential New Biological Roles

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dc.contributor.authorPatricio R. Orrego
dc.contributor.authorMayela Serrano Rodríguez
dc.contributor.authorMauro Cortez
dc.contributor.authorJorge E. Araya
dc.date.accessioned2026-01-06T14:32:07Z
dc.date.available2026-01-06T14:32:07Z
dc.date.issued2021
dc.description.abstractCalcineurin (CaN) is present in all eukaryotic cells, including intracellular trypanosomatid parasites such as Trypanosoma cruzi (Tc) and Leishmania spp. (Lspp). In this study, we performed an in silico analysis of the CaN subunits, comparing them with the human (Hs) and looking their structure, post-translational mechanisms, subcellular distribution, interactors, and secretion potential. The differences in the structure of the domains suggest the existence of regulatory mechanisms and differential activity between these protozoa. Regulatory subunits are partially conserved, showing differences in their Ca2+-binding domains and myristoylation potential compared with human CaN. The subcellular distribution reveals that the catalytic subunits TcCaNA1, TcCaNA2, LsppCaNA1, LsppCaNA1_var, and LsppCaNA2 associate preferentially with the plasma membrane compared with the cytoplasmic location of HsCaNA . For regulatory subunits, HsCaNB-1 and LsppCaNB associate preferentially with the nucleus and cytoplasm, and TcCaNB with chloroplast and cytoplasm. Calpain cleavage sites on CaNA suggest differential processing. CaNA and CaNB of these trypanosomatids have the potential to be secreted and could play a role in remote communication. Therefore, this background can be used to develop new drugs for protozoan pathogens that cause neglected disease.
dc.description.sponsorshipMINEDUC-UA grant ANT1856 FONDECYT-Chile grant 1051045 CeBiB FB-0001, Semillero Grant 5301, Universidad de Antofagasta Áreas de Escaso Desarrollo grant AED 17-18-02. Universidad de Antofagasta Programa de Iniciación en Investigación para Investigadores Jóvenes de la Universidad de Antofagasta (DE446-2015). CONICYT 21150528. São Paulo Research Foundation- FAPESP (Grant number: 2012/24105-3, Grant #2020/13562-0) the National Council for Scientific and Technological Development (CNPq; Grant number 443816/2014-0) Coordination for the Improvement of Higher Education Personnel (CAPES; Finance Code #001). “Discovery of New Antiparasitic Agents” from Union Iberoamericana de Universidades (UIU), Proposal 1054, 1st and 2nd Research Collaboration Fund: 2017–2018 and 2019–2021
dc.identifier.doi10.3390/biom11091322
dc.identifier.issn2218273X
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/595
dc.language.isoes
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceBiomolecules
dc.titleIn Silico Characterization of Calcineurin from Pathogenic Obligate Intracellular Trypanosomatids: Potential New Biological Roles
dc.typeArticle
oaire.citation.volume11
organization.identifier.rorUniversidad de Antofagasta
uantof.identificator.departmentDepartamento de Tecnología Médica
uantof.identificator.facultyFacultad de Ciencias de la Salud
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