Differential Activity and Expression of Proteasome in Seminiferous Epithelium During Mouse Spermatogenesis
| dc.contributor.author | Héctor Zapata Carmona | |
| dc.contributor.author | Emilce Silvina Díaz | |
| dc.contributor.author | Patricio Morales | |
| dc.contributor.author | Marco Jara | |
| dc.date.accessioned | 2026-01-26T15:05:47Z | |
| dc.date.available | 2026-01-26T15:05:47Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Proteasome-mediated protein degradation is essential for maintaining cellular homeostasis, particularly during spermatogenesis, where extensive cellular transformations, such as spermatid differentiation, require precise protein turnover. A key player in this process is the ubiquitin–proteasome system (UPS). This study aimed to investigate proteasome enzymatic activity at different stages of the spermatogenic cycle within the seminiferous tubules of mice and explore the regulatory mechanisms that influence its proteolytic function. Specifically, we assessed the trypsin-like, chymotrypsin-like, and peptidyl-glutamyl-peptide-hydrolyzing (PGPH) activities of the proteasome. Additionally, we examined the expression of catalytic and structural subunits of the 20S core, the assembly of the 20S core with regulatory complexes, and the phosphorylation status of proteasome subunits in various segments of the seminiferous tubules. Our findings demonstrated distinct patterns of proteasomal enzymatic activity in the analyzed segments. While the expression levels of structural and catalytic subunits of the 20S core remained consistent, significant differences were detected in the assembly of the 20S core, the expression of regulatory complexes, and the phosphorylation of proteasome subunits mediated by protein kinase A. These results indicate that proteasomal activity is finely regulated through multiple mechanisms depending on the specific stage of the seminiferous epithelial cycle, highlighting the complexity of proteostasis during spermatogenesis. | |
| dc.description.sponsorship | Fondo Puente de lnvestigación de Excelencia of the University of Antofagasta CR-4496 (P.M.) and FRP22-004 | |
| dc.identifier.doi | 10.3390/ijms26020494 | |
| dc.identifier.issn | 16616596 | |
| dc.identifier.uri | https://repositorioabierto.uantof.cl/handle/uantof/632 | |
| dc.language.iso | en | |
| dc.rights | Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.source | International Journal of Molecular Sciences | |
| dc.title | Differential Activity and Expression of Proteasome in Seminiferous Epithelium During Mouse Spermatogenesis | |
| dc.type | Article | |
| oaire.citation.volume | 26 | |
| organization.identifier.ror | Universidad de Antofagasta | |
| uantof.identificator.department | Departamento Biomédico | |
| uantof.identificator.faculty | Facultad de Ciencias de la Salud | |
| uantof.identificator.institute | Instituto Antofagasta |