Secondary metabolites with antimicrobial activity produced by thermophilic bacteria from a high-altitude hydrothermal system

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dc.contributor.authorPardo Esté, Coral
dc.contributor.authorCortés, Johanna
dc.contributor.authorCastro Severyn, Juan
dc.contributor.authorPérez, Vilma
dc.contributor.authorHenriquez Aedo, Karem
dc.contributor.authorCuadros, Fabian
dc.contributor.authorYañez, Carolina
dc.contributor.authorCuadros Orellana, Sara
dc.contributor.authorDorador, Cristina
dc.contributor.authorMolina, Veronica
dc.contributor.authorEissler, Yoanna
dc.contributor.authorPaquis, Pablo
dc.contributor.authorJeffrey, Wade H.
dc.contributor.authorPozo, Patricia
dc.contributor.authorPérez, Pablo A.
dc.contributor.authorHengst, Martha B.
dc.date.accessioned2026-04-22T20:59:15Z
dc.date.available2026-04-22T20:59:15Z
dc.date.issued2024
dc.description.abstractThermophilic microorganisms possess several adaptations to thrive in high temperature, which is reflected as biosynthesis of proteins and thermostable molecules, isolation and culture represent a great methodological challenge, therefore High throughput sequencing enables screening of the whole bacterial genome for functional potential, providing rapid and cost-effective information to guide targeted cultures for the identification and characterization of novel natural products. In this study, we isolated two thermophilic bacterial strains corresponding to Bacillus LB7 and Streptomyces LB8, from the microbial mats in the Atacama Desert. By combining genome mining, targeted cultures and biochemical characterization, we aimed to identify their capacity to synthesize bioactive compounds with antimicrobial properties. Additionally, we determined the capability to produce bioactive compounds under controlled in vitro assays and detected by determining their masses by Thin-Layer Chromatography/Mass Spectrometry (TLC/MS). Overall, both isolates can produce antimicrobial (e.g., Myxalamide C by-product) and antioxidants (e.g. Dihydroxymandelic Acid, Amide biotine and Flavone by-products) compounds. Bacillus LB7 strain possesses a more diverse repertoire with 51.95% of total metabolites unmatched, while Streptomyces LB8 favors mainly antioxidants, but has over 70% of unclassified compounds, highlighting the necessity to study and elucidate the structure of novel compounds. Based on these results, we postulate that the uncultured or rare cultured thermophiles inhabiting high-altitude hydrothermal ecosystems in the Atacama Desert offer a promising opportunity to the study of novel microbial bioactive compounds.
dc.description.sponsorshipComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT: 1211515, 1181773, 1201692, 1201658, 1171324 Profondecyt-VRIDT UCN ANID 2023 FONDECYT: 3230189, 3210156 ANID: 21221380"
dc.identifier.doi10.3389/fmicb.2024.1477458
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/697
dc.language.isoen
dc.publisherFrontiers Media SA
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceFrontiers in Microbiology
dc.subjectbioprospection
dc.subjectsecondary metabolites
dc.subjectthermophilic bacteria
dc.subjectAntimicrobial activity
dc.subjectbioactive compounds
dc.titleSecondary metabolites with antimicrobial activity produced by thermophilic bacteria from a high-altitude hydrothermal system
dc.typeArticle
oaire.citation.volume15
organization.identifier.rorhttps://ror.org/04eyc6d95
organization.legalNameUniversidad de Antofagasta
uantof.identificator.departmentDepartamento de Biotecnología
uantof.identificator.facultyFacultad de Ciencias del Mar y Recursos Biológicos
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