Integrating Egg Case Morphology and DNA Barcoding to Discriminate South American Catsharks, Schroederichthys bivius and S. chilensis (Carcharhiniformes: Atelomycteridae)

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dc.contributor.authorBustamante, Carlos
dc.contributor.authorVargas-Caro, Carolina
dc.contributor.authorIndurain, Maria J
dc.contributor.authorSilva, Gabriela
dc.date.accessioned2026-08-28T01:47:52Z
dc.date.available2026-08-28T01:47:52Z
dc.date.issued2025
dc.description.abstractCatsharks are benthic elasmobranchs that share spatial niches with littoral and demersal bony fishes. The genus Schroederichthys includes five species, two of which, S. chilensis and S. bivius, occur in the waters of Chile. These species are morphologically similar and are often misidentified because of their overlapping external features and color patterns. To improve species discrimination, we analyzed the egg case morphology of both species based on 36 egg cases (12 S. chilensis, 24 S. bivius) collected from gravid females captured as bycatch in artisanal fisheries between Iquique and Puerto Montt (July–December 2021). Nine morphometric variables were measured and standardized using the total egg case length. Although the egg cases were similar in general appearance, multivariate analyses revealed significant interspecific differences, with egg case height and anterior border width emerging as the most diagnostic variables. Linear discriminant analysis achieved a 100% classification accuracy within this dataset. To confirm species identity, 24 tissue samples (12 per species) were sequenced for the mitochondrial cytochrome c oxidase subunit I (COI) gene. The haplotypes corresponded to previously published sequences from Chile (S. chilensis) and Argentina (S. bivius), with reciprocal monophyly and 100% bootstrap support. While COI barcoding provided robust confirmation, the core contribution of this study lies in the identification of species-specific egg case morphometrics. Together, these findings establish a dual-track toolkit, egg case morphology for primary discrimination and COI barcodes for confirmatory validation, that can be incorporated into bycatch monitoring and biodiversity assessments, supporting the conservation of poorly known catsharks in the Southeast Pacific.
dc.description.sponsorshipAgencia Nacional de Investigación y Desarrollo de Chile (ANID FONDECYT) [11220358]
dc.identifier.doi10.3390/d17090651
dc.identifier.issn1424-2818
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/727
dc.language.isoen
dc.publisherMDPI
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceDiversity
dc.subjectegg capsule
dc.subjectDNA barcoding
dc.subjectpintarroja
dc.subjectoviparous sharks
dc.titleIntegrating Egg Case Morphology and DNA Barcoding to Discriminate South American Catsharks, Schroederichthys bivius and S. chilensis (Carcharhiniformes: Atelomycteridae)
dc.typeArticle
oaire.citation.volume17
oaire.citationIssue9
organization.identifier.rorhttps://ror.org/04eyc6d95
organization.legalNameUniversidad de Antofagasta
uantof.identificator.facultyFacultad de Ciencias del Mar y Recursos Biológicos
uantof.identificator.instituteInstituto de Ciencias Naturales Alexander von Humboldt
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