Uv-a irradiation increases scytonemin biosynthesis in cyanobacteria inhabiting halites at salar grande, atacama desert

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dc.contributor.authorGabriela Orellana
dc.contributor.authorBenito Gómez-Silva
dc.contributor.authorMilton Urrutia
dc.contributor.authorAlexandra Galetović
dc.date.accessioned2025-05-29T13:35:41Z
dc.date.available2025-05-29T13:35:41Z
dc.date.issued2020
dc.description.abstractMicrobial consortia inhabiting evaporitic salt nodules at the Atacama Desert are dominated by unculturable cyanobacteria from the genus Halothece. Halite nodules provide transparency to photosynthetically active radiation and diminish photochemically damaging UV light. Atacama cyanobacteria synthesize scytonemin, a heterocyclic dimer, lipid soluble, UV-filtering pigment (in vivo absorption maximum at 370 nm) that accumulates at the extracellular sheath. Our goal was to demonstrate if UV-A irradiations modulate scytonemin biosynthesis in ground halites containing uncultured Halothece sp. cyanobacteria. Pulverized halite nodules with endolithic colonization were incubated under continuous UV-A radiation (3.6 W/m2) for 96 h, at 67% relative humidity, mimicking their natural habitat. Scytonemin content and relative transcription levels of scyB gene (a key gene in the biosynthesis of scytonemin) were evaluated by spectrophotometry and quantitative RT-PCR, respectively. After 48 h under these experimental conditions, the ratio scytonemin/chlorophyll a and the transcription of scyB gene increased to a maximal 1.7-fold value. Therefore, endolithic Halothece cyanobacteria in halites are metabolically active and UV radiation is an environmental stressor with a positive influence on scyB gene transcription and scytonemin biosynthesis. Endolithobiontic cyanobacteria in Atacama show a resilient evolutive and adaptive strategy to survive in one of the most extreme environments on Earth.
dc.description.sponsorshipSemillero de Investigación, Universidad de Antofagasta (grant number: SI-5305) CONICYT-Chile (grant number: CeBiB FB-0001) Convenio Marco para Universidades Estatales, Ministerio de Educación Chile (grant number: NEXER ANT 1756) IANAS (Inter American Network of Academies of Sciences) Seed Grant.
dc.identifier.doi10.3390/microorganisms8111690
dc.identifier.issn20762607
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/493
dc.language.isoen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceMicroorganisms
dc.titleUv-a irradiation increases scytonemin biosynthesis in cyanobacteria inhabiting halites at salar grande, atacama desert
dc.typeArticle
oaire.citation.volume8
organization.identifier.rorUniversidad de Antofagasta
uantof.identificator.departmentDepartamento Biomédico
uantof.identificator.facultyFacultad de Ciencias de la Salud
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