Bucchi, CristinaGimeno Sandig, ÁlvaroValdivia Gandur, ÍvanCéspedes Manzanares, CristinaMaria de Anta, Josep2026-01-122026-01-1220190258851X10.21873/invivo.11584https://repositorioabierto.uantof.cl/handle/uantof/605Background: This study evaluated the effectiveness of a regenerative endodontic approach to regenerate the pulp tissue in mature teeth of ferret. The presence of odontoblastlike cells in the newly-formed tissue of teeth treated with or without preameloblast-conditioned medium was evaluated based on morphological criteria. Materials and Methods: Twenty-four canines from six ferrets were treated. The pulp was removed, and the apical foramen was enlarged. After inducing the formation of a blood clot, a collagen sponge with or without preameloblast-conditioned medium was placed underneath the cementoenamel junction. The samples were analyzed at the eighth week of follow-up. Results: Vascularized connective tissue was observed in 50% of teeth, without differences between groups. The tissue occupied the apical third of the root canals. Odontoblast-like cells were not observed in any group. Conclusion: Revitalization of mature teeth is possible, at least in the apical third of the root canal. Further experimental research is needed to produce more reliable outcomes.enAttribution-NonCommercial-NoDerivatives 4.0 InternationalArticle