Centro de Bioinnovación
URI permanente para esta colección
Misión
La misión del Centro de Bioinnovación está centrada en construirse como un centro de referencia I+D+i, que liderará investigaciones innovadoras, basadas en el uso de microorganismos con aplicaciones biotecnológicas en la industria regional y nacional, y a su vez promoverá el emprendimiento a través de la generación de productos en el área de la bioinnovación.
Visión
A través del desarrollo, implementación y transferencia de metodologías biotecnológicas innovadoras con base en la utilización de microorganismos como fuentes potenciales de bioproductos, permitirá el desarrollo de actividades económicas basadas en recursos naturales renovables, los cuales permitirán la diversificación de la matriz productiva regional, hasta ahora basada exclusivamente en la minería.
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Examinando Centro de Bioinnovación por Autor "Cameron, Henry"
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Ítem Differentiation of microbial communities in coastal seawater before and during an Akashiwo sanguinea (Dinophyceae) bloom in the urban area of Antofagasta city (northern Chile)(Elsevier, 2025) Hu, Jingming; Cameron, Henry; Rilling, Joaquín I; Campos, Marcos; Ruiz-Gil, Tay; Gonzalez, Mariela A; Gajardo, Gonzalo; Vergara, Karen; Guzmán, Leonardo; Espinoza-González, Oscar; Fuenzalida, Gonzalo; Riquelme, Carlos; Ueki, Shoko; Nagai, Satoshi; Maruyama, Fumito; Fujiyoshi, So; Yarimizu, Kyoko; Perera, Ishara Uhanie; Ávila Barrera, Andrés; Acuña, Jacquelinne J; Zhang, Qian; Jorquera, Milko AHarmful algal blooms (HABs) are highly relevant for public health, the economy, the ecosystem, and biodiversity stability in southern Chile (40° to 53°S), where they occur regularly and are frequently monitored. However, HAB events and their associated microbes in northern Chile (17° to 30°S) remain unknown and difficult to track due to a lack of monitoring, particularly in urban areas. We investigated changes in microbial communities in coastal seawater before and during an Akashiwo sanguinea bloom (B) at two sampling points in Antofagasta city (23°38′39S, 70°24′39W). Seawater samples (filtered at 1 and 0.2 μm) were collected during distinct bloom phases (control, prebloom, and in-bloom), and 16S and 18S rDNA gene libraries were constructed and analyzed via the DNA metabarcoding technique. Our findings indicate that species diversity within the 16S rDNA-based community was greater during the prebloom phase than during the control and in-bloom phases. Conversely, species diversity within the 18S rDNA-based community was lower during the in-bloom phase than during the control and prebloom phases. Independent of the sampling points and fractions, principal coordinate analysis (PCoA) revealed distinct differences in both the 16S and 18S rDNA-based communities between the analyzed bloom phases. Our analysis further revealed that the 16S rDNA-based community was dominated by the Flavobacteriaceae and Rhodobacteraceae families, whose abundance decreased and increased, respectively, during the bloom. As anticipated, the structure of the 18S rDNA-based community was predominantly governed by the Gymnodiniaceae family, specifically the Akashiwo genus, during the bloom. The differentiation in microbial communities was more pronounced in taxa with low abundances than in the dominant taxa. Additionally, co-occurrence network analysis revealed predominantly positive relationships within the microbial communities, particularly during the bloom event. Our analysis also identified several bacterial genera as keystone taxa within the microbial communities, notably members of Pseudomonadota and Bacteroidota. © 2024 Elsevier B.V.Ítem Rapid detection of enterobacteria in wastewater treated by microalgal consortia using loop-mediated isothermal amplification (LAMP)(PeerJ Inc., 2024) Cameron, Henry; Bazaes, Jazmín; Sepúlveda, Claudia; Riquelme, CarlosIn the present study, nine Enterobacteriaceae species present in wastewater were isolated and identified, and loop-mediated isothermal amplification (LAMP) was developed for the detection of Enterobacteriaceae by designing primers based on the mcr-1, KPC, OXA-23, and VIM genes, which are recognized markers of antimicrobial resistance (AMR) transmission during microalgal bioremediation treatment. The developed assays successfully detected four strains positive for mcr-1 gene-asociated resistance (Acinetobacter baylyi, Klebsiella pneumoniae, Morganella morganii, and Serratia liquefaciens), three strains for KPC gene-associated resistance (Acinetobacter sp., Escherichia coli 15499, and Morganella morganii), seven strains for OXA-23 gene-associated resistance (Acinetobacter baylyi, Enterobacter hormaechi, Enterobacter cloacae, Escherichia coli 15922, Escherichia coli 51446, Morganella morganii, and Serratia liquefaciens), and three strains for resistance to the VIM gene-associated resistance (Acinetobacter baylyi, Acinetobacter sp., and Enterobacter hormaechi) from a single colony. A reduction in microbiological load of 93.6% was achieved at 15 colony-forming units (CFU) mL-1, utilizing EMB agar and LAMP values of 0.142 +/- 0.011 for the mcr-1 gene, 0.212 +/- 0.02 for the KPC gene, 0.233 +/- 0.006 for the OXA-23 gene, and 0.219 +/- 0.035 for the VIM gene. Furthermore, bioremediation efficiency values of 71.6% and 75% for total nitrogen and phosphorus, respectively, were observed at 72 h of treatment in open pond microalgal remediation systems (MRS). This study demonstrated that the LAMP technique is faster and more sensitive than traditional detection methods, such as CFU, for Enterobacteriaceae. Consequently, this method may be considered for the detection of microbiological quality indicators within the water treatment industry.Ítem Sustainable microalgal feed formulations for functional and omega-3-enriched eggs in poultry production(Elsevier B.V., 2026) Diaz Mac Adoo , Daniela; Mata, María Teresa; Kaltsidi, Maria P.; Ardiles, Paula; Jensen, Niel; Cruz-Balladares, Victoria; Cameron, Henry; Avalos, Vladimir; Sepúlveda-Sánchez, Francisco; Palma, Jennifer; Riquelme, CarlosThis study evaluated the dietary supplementation of Hy-Line Brown® laying hens with a microalgal blend composed of Microchloropsis gaditana and Muriellopsis sp. strain MCH-35 at inclusion levels of 1% and 3% (microalgal mix) and 10% (M. gaditana), aiming to improve the nutritional and functional quality of eggs. The formulated pellets were analyzed for proximate composition, fatty acid profile, carotenoid content, and antioxidant capacity, while the feeding trials were conducted over eight weeks. Microalgal inclusion enhanced the nutritional profile of the feed by increasing protein, lipid, and mineral contents while reducing crude fiber. M. gaditana contributed high levels of eicosapentaenoic acid (EPA), whereas Muriellopsis sp. exhibited higher lutein and total carotenoid contents, significantly increasing antioxidant capacity. In egg yolks, supplementation produced a progressive, dose-dependent increase in pigmentation, with the most intense coloration observed in the 3% and 10% treatments. Additionally, a significant increase in docosahexaenoic acid (DHA) and a reduction in linoleic acid (n-6) were recorded, improving the n-6/n-3 ratio from 24:1 (control) to 7:1 (3%) and 6:1 (10% M. gaditana). Total carotenoid and lutein contents in yolks increased by 2.3- and 2.7-fold, respectively, without affecting egg physical parameters. Biochemical profiles of the hens confirmed the absence of hepatic or metabolic stress, validating the safety of supplementation. Microalgae-enriched pellets offer an innovative and sustainable feed alternative that enables the production of functional eggs enriched with omega-3 fatty acids and carotenoids, enhancing their nutritional quality while adding both economic and environmental value to the poultry sector.