Centro de Bioinnovación

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https://repositorioabierto.uantof.cl/handle/uantof/495
MISIÓN La misión del Centro de bioinnovación está centrada en construirse como un centro de referencia I+D+i, que liderará investigaciones innovadoras, basadas en el uso de microorganismos con aplicaciones biotecnológicas en la industria regional y nacional, y a su vez promoverá el emprendimiento a través de la generación de productos en el área de la bioinnovación. VISIÓN A través del desarrollo, implementación y transferencia de metodologías biotecnológicas innovadoras con base en la utilización de microorganismos como fuentes potenciales de bioproductos. permitirá el desarrollo de actividades económicas basadas en recursos naturales renovables, los cuales permitirán la diversificación de la matriz productiva regional, hasta ahora basada exclusivamente en la minería. https://www.centrodebioinnovacion-ua.com/ https://cdeaua.cl/home/virtual/cbia/

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  • Miniatura
    Ítem
    Sulfate Nutrition Modulates the Oxidative Response against Short-Term Al3+-Toxicity Stress in Lolium perenne cv. Jumbo Shoot Tissues
    (2024) Hernan Vera Villalobos; Lizzeth Lunario Delgado; Anita S. Gálvez; Domingo Román Silva; Ana Mercado Seguel; Cristián Wulff Zottele
    Al3+-toxicity in acidic soils is among the main abiotic stress factors that generate adverse effects in plant growth; in leaves, it affects several physiological parameters such as photosynthesis and ROS balance, leading to limited crop production. On the other hand, sulfur is a macronutrient that has a key role against oxidative stress and improves plant growth in acidic soils; however, the implication of sulfate nutritional status in the modulation of short-term Al3+-toxicity tolerance mechanisms in plant leaves are barely reported. This study is focused on the role of sulfate on the leaf response of an Al3-sensitive perennial ryegrass (Lolium perenne cv. Jumbo) after 48 h of exposure. Lolium perenne cv. Jumbo seeds were cultivated in hydroponic conditions with modified Taylor Foy solutions supplemented with 120, 240, and 360 μM sulfate in the presence or absence of Al3+-toxicity. The L. perenne cv. Jumbo leaves were collected after 48 h of Al3+-toxicity exposure and processed to evaluate the effects of sulfate on Al3+ toxicity, measuring total proteins, mineral uptake, photosynthesis modulation, and ROS defense mechanism activation. The plants exposed to Al3+-toxicity and cultivated with a 240 μM sulfate amendment showed a recovery of total proteins and Ca2+ and Mg2+ concentration levels and a reduction in TBARS, along with no changes in the chlorophyll A/B ratio, gene expression of proteins related to photosynthesis (Rubisco, ChlAbp, and Fered), or ROS defense mechanism (SOD, APX, GR, and CAT) as compared with their respective controls and the other sulfate conditions (120 and 360 μM). The present study demonstrates that adequate sulfate amendments have a key role in regulating the physiological response against the stress caused by Al3+ toxicity.
  • Miniatura
    Ítem
    Rapid detection of enterobacteria inwastewater treated by microalgal consortiausing loop-mediated isothermalamplification (LAMP)
    (2024) Henry Cameron; Jazmín Bazaes; Claudia Sepúlveda; Carlos Riquelme
    In the present study, nine Enterobacteriaceae species present in wastewater were isolated and identified, and loop-mediated isothermal amplification (LAMP) was developed for the detection of Enterobacteriaceae by designing primers based on themcr-1, KPC, OXA-23, and VIM genes, which are recognized markers of antimicrobial resistance (AMR) transmission during microalgal bioremediation treatment. The developed assays successfully detected four strains positive for mcr-1gene-asociated resistance (Acinetobacter baylyi,Klebsiella pneumoniae,Morganellamorganii, andSerratia liquefaciens), three strains for KPC gene-associated resistance(Acinetobacter sp.,Escherichia coli15499, and Morganella morganii), seven strains for OXA-23 gene-associated resistance (Acinetobacter baylyi,Enterobacterhormaechi,Enterobacter cloacae,Escherichia coli15922,Escherichia coli51446,Morganella morganii, and Serratia liquefaciens), and three strains for resistance to the VIM gene-associated resistance (Acinetobacter baylyi,Acinetobactersp., and Enterobacter hormaechi) from a single colony. A reduction in microbiological load of93.6% was achieved at 15 colony-forming units (CFU) mL-1, utilizing EMB agar andLAMP values of 0.142 ± 0.011 for the mcr-1 gene, 0.212 ± 0.02 for the KPC gene,0.233 ± 0.006 for the OXA-23 gene, and 0.219 ± 0.035 for the VIM gene.Furthermore, bioremediation efficiency values of 71.6% and 75% for total nitrogen and phosphorus, respectively, were observed at 72 h of treatment in open pond microalgal remediation systems (MRS). This study demonstrated that the LAMP technique is faster and more sensitive than traditional detection methods, such as CFU, for Enterobacteriaceae. Consequently, this method may be considered for the detection of microbiological quality indicators within the water treatment industry
  • Miniatura
    Ítem
    Nitrification characteristics and microbial community changes during conversion of freshwater to seawater in down-flow hanging sponge reactor
    (2024) Takumi Akamine; Mami Nagai; Takahiro Watari; Hirotoshi Netsu; Nur Adlin; Penpicha Satanwat; Carlos Riquelme; Masashi Hatamoto; Takashi Yamaguchi
    In recirculating aquaculture systems (RAS), maintaining water quality in aquaculture tanks is a paramount factor for effective fish production. A down-flow hanging sponge (DHS) reactor, a trickling filter system used for water treatment of RAS that employs sponges to retain biomass, has high nitrification activity. However, nitrification in seawater RAS requires a long start-up time owing to the high salinity stress. Therefore, this study aimed to evaluate the nitrification characteristics and changes in the microbial community during the conversion of freshwater to seawater in a DHSreactor fed with ammonia-based artificial seawater. The total ammonia nitrogen concentration reached 1.0 mg-N⋅L 1 (initial concentration 10 mg-N⋅L 1) within 11 days of operation, and nitrate production was observed. The 16 S rRNA gene sequence of the DHS-retained sludge indicated that the detection rate of the ammonia-oxidizing archaeon Candidatus Nitrosocosmicus decreased from 23.9 % to 14.0 % and 25.8–17.6 % in the upper and lower parts of the DHS reactor, respectively, after the introduction of seawater. In contrast, the nitrite-oxidizing bacteria Nitrospira spp. increased from 0.1 % to 9.5 % and from 0.5 % to 10.5 %, respectively. The ammonia oxidation rates of 0.12 ±0.064 and 0.051 ±0.0043 mg-N⋅g-MLVSS 1⋅h 1 on the 37th day in the upper and bottom layers, respectively. Thus, nitrification in the DHS reactor performed well, even under high-salinity conditions with short operational days. This finding makes the transition from freshwater to saltwater fish in the RAS system simple and economical, and has the potential for early start-up of the RAS.
  • Miniatura
    Ítem
    Insights into the diversity and survival strategies of soil bacterial isolates from the Atacama Desert
    (2024) Alicyn Reverdy; Daniel Hathaway; Jessica Jha; Gabriel Michaels; Jeffrey Sullivan; Daniela Diaz McAdoo; Carlos Riquelme; Yunrong Chai; Veronica Godoy Carter
    The Atacama Desert, the driest, with the highest radiation, and one of the most ancient deserts in the world, is a hostile environment for life. We have a collection of 74 unique bacterial isolates after cultivation and confirmation by 16S rRNA gene sequencing. Pigmentation, biofilm formation, antimicrobial production against Escherichia coli MG1655 and Staphylococcus aureus HG003,and antibiotic resistance were assessed on these isolates. We found that approximately a third of the colonies produced pigments, 80% of isolates formed biofilms, many isolates produce growth inhibiting activities against E. coliand/orS. aureus, and many were resistant to antibiotics. The functional characterization of these isolates gives us insight into the adaptive bacterial strategies in harsh environments and enables us to learn about their possible use in agriculture,healthcare, or biotechnology.
  • No hay miniatura disponible
    Ítem
    Rapid detection of enterobacteria in wastewater treated by microalgal consortia using loop-mediated isothermal amplification (LAMP)
    (2024) Henry Cameron; Jazmín Bazaes; Claudia Sepúlveda; Carlos Riquelme
    In the present study, nine Enterobacteriaceae species present in wastewater were isolated and identified, and loop-mediated isothermal amplification (LAMP) was developed for the detection of Enterobacteriaceae by designing primers based on the mcr-1, KPC, OXA-23, and VIM genes, which are recognized markers of antimicrobial resistance (AMR) transmission during microalgal bioremediation treatment. The developed assays successfully detected four strains positive for mcr-1 gene-asociated resistance (Acinetobacter baylyi, Klebsiella pneumoniae, Morganella morganii, and Serratia liquefaciens), three strains for KPC gene-associated resistance (Acinetobacter sp., Escherichia coli 15499, and Morganella morganii), seven strains for OXA-23 gene-associated resistance (Acinetobacter baylyi, Enterobacter hormaechi, Enterobacter cloacae, Escherichia coli 15922, Escherichia coli 51446, Morganella morganii, and Serratia liquefaciens), and three strains for resistance to the VIM gene-associated resistance (Acinetobacter baylyi, Acinetobacter sp., and Enterobacter hormaechi) from a single colony. A reduction in microbiological load of 93.6% was achieved at 15 colony-forming units (CFU) mL-1, utilizing EMB agar and LAMP values of 0.142 +/- 0.011 for the mcr-1 gene, 0.212 +/- 0.02 for the KPC gene, 0.233 +/- 0.006 for the OXA-23 gene, and 0.219 +/- 0.035 for the VIM gene. Furthermore, bioremediation efficiency values of 71.6% and 75% for total nitrogen and phosphorus, respectively, were observed at 72 h of treatment in open pond microalgal remediation systems (MRS). This study demonstrated that the LAMP technique is faster and more sensitive than traditional detection methods, such as CFU, for Enterobacteriaceae. Consequently, this method may be considered for the detection of microbiological quality indicators within the water treatment industry.