Departamento de Biotecnología

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    Ítem
    Inactivated Vaccine-Induced SARS-CoV-2 Variant-SpecificImmunity in Children
    (2022) Jorge A. Soto; Felipe Melo González; Cristián Gutierrez Vera; Bárbara M. Schultz; Roslye V. Berríos Rojas; Daniela Rivera Pérez; Alejandro Piña Iturbe; Guillermo Hoppe Elsholz; Luisa F. Duarte; Yaneisi Vázquez; Daniela Moreno Tapia; Mariana Ríos; Pablo A. Palacios; Richard Garcia Betancourt; Álvaro Santibañez; Gaspar A. Pacheco; Constanza Mendez; Catalina A. Andrade; Pedro H. Silva; Benjamín Diethelm Varela; Patricia Astudillo; Mario Calvo; Antonio Cárdenas; Marcela González; Macarena Goldsack; Valentina Gutiérrez; Marcela Potin; Andrea Schilling; Lorena I. Tapia; Loreto Twele; Rodolfo Villena; Alba Grifoni; Alessandro Sette; Daniela Weiskopf; Rodrigo A. Fasce; Jorge Fernández; Judith Mora; Eugenio Ramírez; Aracelly Gaete Argel; Mónica L. Acevedo; Fernando Valiente Echeverría; Ricardo Soto Rifo; Angello Retamal Díaz; Nathalia Muñoz Jofré; PedCoronaVac03CL Study Group; Xing Meng; Qianqian Xin; Eduardo Alarcón Bustamante; José V. González Aramundiz; Nicole Le Corre; María Javiera Álvarez Figueroa; Pablo A. González; Katia Abarca; Cecilia Perret; Leandro J. Carreño; Susan M. Bueno; Alexis M. Kalergis
    Multiple vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been evaluated in clinical trials. However, trials addressing the immune response in the pediatric population are scarce. The inactivated vaccine CoronaVac has been shown to be safe and immunogenic in a phase 1/2 clinical trial in a pediatric cohort in China. Here, we report interim safety and immunogenicity results of a phase 3 clinical trial for CoronaVac in healthy children and adolescents in Chile. Participants 3 to 17 years old received two doses of CoronaVac in a 4-week interval until 31 December 2021. Local and systemic adverse reactions were registered for volunteers who received one or two doses of CoronaVac. Whole-blood samples were collected from a subgroup of 148 participants for humoral and cellular immunity analyses. The main adverse reaction reported after the first and second doses was pain at the injection site. Four weeks after the second dose, an increase in neutralizing antibody titer was observed in subjects relative to their baseline visit. Similar results were found for activation of specific CD41 T cells. Neutralizing antibodies were identified against the Delta and Omicron variants. However, these titers were lower than those for the D614G strain. Importantly, comparable CD41 T cell responses were detected against these variants of concern. Therefore, CoronaVac is safe and immunogenic in subjects 3 to 17 years old, inducing neutralizing antibody secretion and activating CD41 T cells against SARS-CoV-2 and its variants. (This study has been registered at ClinicalTrials .gov under no. NCT04992260.)
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    Ítem
    Response to static magnetic field-induced stress in scenedesmus obliquus and nannochloropsis gaditana
    (2021) Génesis Serrano; Carol Miranda-Ostoijc; Pablo Ferrada; Cristian Wulff-Zotelle; Alejandro Maureira; Edward Fuentealba; Karem Gallardo; Manuel Zapata; Mariella Rivas
    Magnetic fields in biological systems is a promising research field; however, their application for microalgae has not been fully exploited. This work aims to measure the enzymatic activity and non-enzymatic activity of two microalgae species in terms of superoxide dismutase (SOD), catalase (CAT), and carotenoids, respectively, in response to static magnetic fields-induced stress. Two magnet configurations (north and south) and two exposure modes (continuous and pulse) were applied. Two microalgae species were considered, the Scenedesmus obliquus and Nannochloropsis gaditana. The SOD activity increased by up to 60% in S. obliquus under continuous exposure. This trend was also found for CAT in the continuous mode. Conversely, under the pulse mode, its response was hampered as the SOD and CAT were reduced. For N. gaditana, SOD increased by up to 62% with the south configuration under continuous exposure. In terms of CAT, there was a higher activity of up to 19%. Under the pulsed exposure, SOD activity was up to 115%. The CAT in this microalga was increased by up to 29%. For N. gaditana, a significant increase of over 40% in violaxanthin production was obtained compared to the control, when the microalgae were exposed to SMF as a pulse. Depending on the exposure mode and species, this methodology can be used to produce oxidative stress and obtain an inhibitory or enhanced response in addition to the significant increase in the production of antioxidant pigments.