Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides

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dc.contributor.authorAlexandra Galetović
dc.contributor.authorGabriel Peña
dc.contributor.authorNicole Fernández
dc.contributor.authorMilton Urrutia
dc.contributor.authorNataly Flores
dc.contributor.authorBenito Gómez-Silva
dc.contributor.authorJocelyne Di Ruggiero
dc.contributor.authorCarolina Shene
dc.contributor.authorMariela Bustamante
dc.date.accessioned2025-10-21T12:39:50Z
dc.date.available2025-10-21T12:39:50Z
dc.date.issued2023
dc.description.abstractCyanobacteria produce exopolysaccharides (EPSs) as an adaptative mechanism against ultraviolet radiation and desiccation. Cellulose is present in the extracellular polymeric substance in some cyanobacteria genera and it has been proposed as a raw material for biofuel production. The goal of this work was to evaluate the cellulose presence in EPS of Atacama cyanobacteria strains and its use as an alternative and innovative biological source to produce bioethanol. The presence of cellulose was evaluated using techniques of molecular biology, bioinformatics, and electronic microscopy. The conserved motif D,D,D,35QXXRW, characteristic of processive -glycosyltransferase in all cellulose-producing organisms, was identified in the genome of the LLA-10 strain. This is evidence that cellulose synthase in the LLA-10 strain is a functional enzyme. EPS from Atacama cyanobacteria was hydrolyzed by -glucosidases (cellobiase and cellulase) and the released glucose was yeast-fermented to ethanol. Ethanol production reached 172.69 0.02 mg ethanol/g EPS after 48 h of incubation. These results are the first step in the evaluation of EPS produced by native cyanobacteria isolated from northern Chile for future biotechnological applications such as the production of bioethanol.
dc.description.sponsorshipFondo de Innovación para la Competitividad Regional (FIC-R) (grant number: 4603) IANAS (Interamerican Network of Academies of Sciences) Fellowships Award. John Hopkins University, USA; IANAS Seed Grant National Academy of Science, USA; Semillero de Investigación, Universidad de Antofagasta (grant number: SI-5305) ANID, Millennium Science Initiative Program—ICN2021_044 Centre for Biotechnology and Bioengineering (grant number: CeBiB FB-0001) Financiamiento ANT20992 para Asistente de Investigación “Fortalecimiento de la investigación, innovación y posgrado de la Universidad de Antofagasta, enfatizando la asociatividad con alianzas y programas de posgrado que impacten en las necesidades regionales”
dc.identifier.doi10.3390/microorganisms11112668
dc.identifier.issn2076-2607
dc.identifier.urihttps://repositorioabierto.uantof.cl/handle/uantof/580
dc.language.isoen
dc.sourcemicroorganisms
dc.titleCellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides
dc.typeArticle
oaire.citation.volume11
organization.identifier.rorUniversidad de Antofagasta
uantof.identificator.departmentDepartamento Ciencias Médicas
uantof.identificator.departmentDepartamento Biomédico
uantof.identificator.facultyFacultad de Medicina y Odontología
uantof.identificator.facultyFacultad de Ciencias de la Salud
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